LCMS ANALYSIS OF ROSELLA EXTRACTS (HIBISCUS SABDARIFFA LINN)
Abstract
Rosella (Hibiscus sabdariffa Linn) is a traditional medicinal plant with more than 300 species distributed in tropical and subtropical regions. The plant is grown for its fibre as a medicinal ingredient. Rosella has various benefits due to its diverse chemical compounds, such as alkaloids, anthocyanins, flavonoids, tannins, steroids, and sterols. This study discussed the potential of rosella as an anti-inflammatory, antioxidant, antibacterial, antihypertensive, antidiabetic, and anticholesterol. To observe the chemical compound content of rosella flower extract. Methods: LCMS (Liquid Chromatography-Mass Spectrometry) test to see the content of chemical compounds in rosella flower extract. LCMS test showed that rosella flower extract contains flavonoids, tannins, phenolic-quinic acid, phenolic, and alkaloid chemical compounds. Rosella flower extract contains chemical compounds such as flavonoids, tannins, phenolics, quinic acid, and alkaloids, which have antibacterial, antioxidant, anti-inflammatory properties, as well as diuretic, anticholesterol, antidiabetic, and antihypertensive effects. These compounds give rosella great potential as a natural ingredient with various pharmacological applications. Although the results showed that rosella has many pharmacological benefits, further research is still needed to understand the deeper mechanisms and potential clinical use of rosella for the treatment of various diseases, especially in dentistry.
References
Abedi, F., Razavi, B. M., & Hosseinzadeh, H. (2020). A review on gentisic acid as a plant derived phenolic acid and metabolite of aspirin: Comprehensive pharmacology, toxicology, and some pharmaceutical aspects. Phytotherapy Research, 34(4), 729–741. https://doi.org/10.1002/ptr.6573
Amriani, Y. A., & Tuahatu, J. W. (2021). Uji aktivitas antioksidan ekstrak etanol-air (1: 1) bunga rosella (Hibiscus sabdariffa L.) dengan metode DPPH (1, 1-difenil-2-pikrilhidrazil). Jurnal Penelitian Sains, 21(3), 163–167.
Aritonang, T. R., Siantar, R. L., & Simanjuntak, F. M. (2021). The Effectiveness of Steeping Rosella (Hibiscus Sabdariffa) Against Hypertension in the Elderly. International Journal of Science and Society, 3(1), 412–419. https://doi.org/10.54783/ijsoc.v3i1.308
Arowoogun, J., Akanni, O. O., Adefisan, A. O., Owumi, S. E., Tijani, A. S., & Adaramoye, O. A. (2021). Rutin ameliorates copper sulfate-induced brain damage via antioxidative and anti-inflammatory activities in rats. Journal of Biochemical and Molecular Toxicology, 35(1), 1–8. https://doi.org/10.1002/jbt.22623
Asworo, R. Y., & Widwiastuti, H. (2023). Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak. Indonesian Journal of Pharmaceutical Education, 3(2), 256–263. https://doi.org/10.37311/ijpe.v3i2.19906
Badaring, D. R., Sari, S. P. M., Nurhabiba, S., Wulan, W., & Lembang, S. A. R. (2020). Uji Ekstrak Daun Maja (Aegle marmelos L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. Indonesian Journal of Fundamental Sciences, 6(1), 16. https://doi.org/10.26858/ijfs.v6i1.13941
Chaudhari, H. C., & Chavan, G. M. (2022). a Brief Review on Hibiscus Sabdariffa Linn With Their Medicinal Uses and Pharmacological Activity. International Journal of Pharmaceutical Sciences and Research, 13(8), 3112–3127. https://doi.org/10.13040/IJPSR.0975-8232.13(8).3112-27
Che, J., Zhao, T., Liu, W., Chen, S., Yang, G., Li, X., & Liu, D. (2021). Neochlorogenic acid enhances the antitumor effects of pingyangmycin via regulating TOP2A. Molecular Medicine Reports, 23(2), 1–9. https://doi.org/10.3892/mmr.2020.11797
Chen, X., Ying, X., Sun, W., Zhu, H., Jiang, X., & Chen, B. (2018). The therapeutic effect of fraxetin on ethanol-induced hepatic fibrosis by enhancing ethanol metabolism, inhibiting oxidative stress and modulating inflammatory mediators in rats. International Immunopharmacology, 56(January), 98–104. https://doi.org/10.1016/j.intimp.2018.01.027
Da-Costa-Rocha, I., Bonnlaender, B., Sievers, H., Pischel, I., & Heinrich, M. (2014). Hibiscus sabdariffa L. - A phytochemical and pharmacological review. Food Chemistry, 165, 424–443. https://doi.org/10.1016/j.foodchem.2014.05.002
Deng, S.-J., Ge, J.-W., Xia, S.-N., Zou, X.-X., Bao, X.-Y., Gu, Y., Xu, Y., & Meng, H.-L. (2022). Fraxetin alleviates microglia-mediated neuroinflammation after ischemic stroke. Annals of Translational Medicine, 10(8), 439–439. https://doi.org/10.21037/atm-21-4636
Diyah, N. W., Nasyanska, A. L., & Purwanto, B. T. (2020). Analgesic Activity of Acyl-Salicylic Acid Derivatives And In Silico Docking Study For Their Potency As Cyclooxygenase-2 Inhibitors. Pharmacy UMG, 7(2), 47–54.
Ekeuku, S. O., Pang, K. L., & Chin, K. Y. (2021). Effects of caffeic acid and its derivatives on bone: A systematic review. Drug Design, Development and Therapy, 15, 259–275. https://doi.org/10.2147/DDDT.S287280
Fatma, M., Chatri, M., Fifendy, M., & Handayani, D. (2021). Effect of Papaya Leaf Extract (Carica papaya L.) on Colony Diameter and Percentage of Growth Inhibition of Fusarium oxysporum Pengaruh Ekstrak Daun Pepaya (Carica papaya L.) terhadap Fusarium oxysporum. Serambi Biologi, 6(2), 9–14.
Fitriaturosidah, I., Kusnadi, J., Nurnasari, E., Nurindah, & Hariyono, B. (2022). Parameter Spesifik dan Non Spesifik Seduhan Herbal Celup Kelopak Bunga Rosella (Hibiscus Sabdariffa) dengan Penambahan Daun Pandan Wangi (Pandanus Amaryllifolius) dan Kayu Manis (Cinnamomum Burmanii). IOP Conference Series: Earth and Environmental Science, 974(1). https://doi.org/10.1088/1755-1315/974/1/012118
Gąsowska-Bajger, B., Sosnowska, K., Gąsowska-Bodnar, A., & Bodnar, L. (2023). The Effect of Acetylsalicylic Acid, as a Representative Non-Steroidal Anti-Inflammatory Drug, on the Activity of Myeloperoxidase. Pharmaceuticals, 16(7). https://doi.org/10.3390/ph16071012
Ghani, B. A., Sc, M., & Ph, D. (2018). Effect of Topical Application Effect of Topical Application of Flavanoids Extract of Hibiscus Sabdariffa on Experimentally Induced Bone Defect. 30(March), 33–38.
Hakim, A. R., Nayaken, P. O., & Alawiyah, T. (2023). Pengaruh Metode Ekstraksi terhadap Kadar Alkaloid Total Ekstrak Daun Kirinyuh (Chromolaena odorata). Indonesian Journal of Pharmacy and Natural Product, 6(02), 194–200. https://doi.org/10.35473/ijpnp.v6i02.2508
Ikrawan, Y., & Muntaha, F. M. (2020). ANALISIS BUNGA TELANG ( Clitoria ternatea ) DENGAN VARIASI Ph METODE LIQUID CHROMATOGRAPH-TANDEM MASS SPECTROMETRY ( LC-MS / MS ). Pasundan Food Technology Journal, 7(2), 70–77. https://journal.unpas.ac.id
Khan, M. (2017). Nutritional and Health Importance of Hibiscus Sabdariffa: A Review and Indication for Research Needs. Journal of Nutritional Health & Food Engineering, 6(5). https://doi.org/10.15406/jnhfe.2017.06.00212
Kurniawati, I., Maftuch, & Hariati, A. M. (2016). PENENTUAN PELARUT DAN LAMA EKSTRAKSI TERBAIK PADA TEKNIK MASERASI Gracilaria sp. SERTA PENGARUHNYA TERHADAP KADAR AIR DAN RENDEMEN. Samakia: Jurnal Ilmu Perikanan, 7(2), 72–77. http://www.samakia.aperiki.ac.id/index.php/JSAPI/article/view/106
Kusparmanto, F. S., Pakpahan, E. L., & ... (2024). Study in the Potential of Chemical Compounds of Rosella Flower (Hibiscus Sabdariffa Linn) Extract As a Prevention of Relapse After Orthodontic Treatment. … Review on Social …, 4(1), 78–87. https://doi.org/10.32509/mirshus.v4i1.71
Kusumastuti, E., Handajani, J., & Susilowati, H. (2014). Ekspresi COX-2 dan Jumlah Neutrofil Fase Inflamasi pada Proses Penyembuhan Luka Setelah Pemberian Sistemik Ekstrak Etanolik Rosela (Hibiscus sabdariffa) (studi in vivo pada Tikus Wistar). Majalah Kedokteran Gigi Indonesia, 21(1), 13. https://doi.org/10.22146/majkedgiind.8778
Lee, S., Lee, J., Lee, H., & Sung, J. (2019). Relative protective activities of quercetin, quercetin-3-glucoside, and rutin in alcohol-induced liver injury. Journal of Food Biochemistry, 43(11), 1–9. https://doi.org/10.1111/jfbc.13002
Lestari, G. A. D. (2022). SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BUNGA ROSELLA UNGU (Hibiscus sabdariffa L.). Jambura Journal of Chemistry, 4(1), 17–24. https://doi.org/10.34312/jambchem.v4i1.11157
Lestari, L., Ata, P. F., Yulianti, A. D., Hasan, H., Cahyo, R. N., Rahman, Z. A., Rahmadani, A., & Erika, F. (2023). PENENTUAN KADAR FENOLIK DAN FLAVONOID TOTAL PADA BUAH KELAPA SAWIT (Elais guineensis Jacq) DENGAN METODE SPEKTROFOTOMETRI UV-VIS. Lantanida Journal, 11(2), 158. https://doi.org/10.22373/lj.v11i2.19676
Maisarah, M., Chatri, M., & Advinda, L. (2023). Karakteristik dan Fungsi Senyawa Alkaloid sebagai Antifungi pada Tumbuhan. Jurnal Serambi Biologi, 8(2), 231–236.
Majeed, S. S., & Ghani, B. A. (2018). Effect of Topical Application of Flavonoids Extract of Hibiscus Sabdariffa on Experimentally Induced Bone Defect. Journal of Baghdad College of Dentistry, 30(1), 33–38. https://doi.org/10.12816/0046309
Mangurana, W. O. I., Yusnaini, Y., & Sahidin, S. (2019). ANALISIS LC-MS/MS (Liquid Crhomatogaph Mass Spectrometry) DAN METABOLIT SEKUNDER SERTA POTENSI ANTIBAKTERI EKSTRAK n-HEKSANA SPONS Callyspongia aerizusa YANG DIAMBIL PADA KONDISI TUTUPAN TERUMBU KARANG YANG BERBEDA DI PERAIRAN TELUK STARING. Jurnal Biologi Tropis, 19(2), 131–141. https://doi.org/10.29303/jbt.v19i2.1126
Nicolin, V., De Tommasi, N., Nori, S. L., Costantinides, F., Berton, F., & Di Lenarda, R. (2019). Modulatory effects of plant polyphenols on bone remodeling: A prospective view from the bench to bedside. Frontiers in Endocrinology, 10(July), 1–9. https://doi.org/10.3389/fendo.2019.00494
Nurnasari, E., & Khuluq, A. D. (2018). Potensi Diversifikasi Rosela Herbal (Hibiscus sabdariffa L.) untuk Pangan dan Kesehatan. Buletin Tanaman Tembakau, Serat & Minyak Industri, 9(2), 82. https://doi.org/10.21082/btsm.v9n2.2017.82-92
Obouayeba, A. P., Djyh, N. B., Diabate, S., Djaman, A. J., N’Guessan, J. D., Kone, M., & Kouakou, T. H. (2014). Phytochemical and antioxidant activity of roselle (Hibiscus Sabdariffa L.) petal extracts. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5(2), 1453–1465.
Oresajo, C. (2020). MANFAAT LARUTAN VITAMIN C DAN VITAMIN E YANG DISTABILKAN FERULIC ACID PADA KULIT MANUSIA. Journal Medika Utama, 02(01), 402–406.
Ramadhani, F. A., Prastika, M. F., Fikriyah, N., Isnaeni, & Diyah, N. W. (2024). Molecular Docking of Flavonoids from Extract of Roselle (Hibiscus sabdariffa L.) Calyx on PBP2a as the Basis for Antibacterial Activity Against Methicillin Resistant Staphylococcus aureus. Science and Technology Indonesia, 9(2), 487–493. https://doi.org/10.26554/sti.2024.9.2.487-493
Silva, H., & Lopes, N. M. F. (2020). Cardiovascular Effects of Caffeic Acid and Its Derivatives: A Comprehensive Review. Frontiers in Physiology, 11(November), 1–20. https://doi.org/10.3389/fphys.2020.595516
Sukmana, B. I., Budhy, T. I., & Ardani, I. G. A. W. (2017). The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction. Dental Journal (Majalah Kedokteran Gigi), 50(1), 36. https://doi.org/10.20473/j.djmkg.v50.i1.p36-42
Copyright (c) 2025 Moestopo International Review on Social, Humanities, and Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
